Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.
| Names | |
|---|---|
| IUPAC name Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate | |
| Other names 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt Amidoschwarz Naphthol blue black Acid Black 1 Acidal Black 10B Acidal Navy Blue 3BR Naphthalene Black 10B Buffalo Black NBR C.I. 20470 |
Identifiers
CAS Number
1064-48-8
3D model (JSmol)
Interactive imageChEBI
CHEBI:86230ChemSpider
10660833ECHA InfoCard100.012.640 
EC Number
213-903-1
PubChem CID
9566044UNII
SZT789770M
CompTox Dashboard(EPA)
DTXSID1024415
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InChI
InChI=1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
Key: AOMZHDJXSYHPKS-DROYEMJCSA-L
InChI=1/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
Key: AOMZHDJXSYHPKS-NJAWIMKDBA
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SMILES
[Na+].[Na+].O=N(=O)c1ccc(cc1)/N=N/c2c(N)c4c(cc2S([O-])(=O)=O)cc(c(/N=N/c3ccccc3)c4O)S([O-])(=O)=OProperties C22H14N6Na2O9S2 Molar mass 616.49 g·mol−1 Appearance Dark red to black ~ 30 g/l at 20 °C (68 °F) Hazards GHS pictograms 
GHS Signal word Warning NFPA 704(fire diamond) Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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