A random hexamer or random hexonucleotides are for various PCR applications such as rolling circle amplification to prime the DNA.
They are oligonucleotide sequences of 6 bases which are synthesised entirely randomly to give a numerous range of sequences that have the potential to anneal at many random points on a DNA sequence and act as a primer to commence first strand cDNA synthesis. [1][2][3]
References[edit]
- ^ http://www.invitrogen.com/site/us/en/home/References/protocols/nucleic-acid-amplification-and-expression-profiling/pcr-protocol/pcr-and-rt-pcr.html
- ^ https://www.thermoscientificbio.com/general-reagents-and-accessories/primers-for-cdna-synthesis
- ^ Hansen, KD; Brenner, SE; Dudoit, S (2010). "Biases in Illumina transcriptome sequencing caused by random hexamer priming". Nucleic Acids Res. 38: e131. doi:10.1093/nar/gkq224. PMC 2896536. PMID 20395217.
Sunday, September 12, 2021
09-11-2021-2203 - Rhodocene bis(cyclopentadienyl)rhodium(II) dicyclopentadienylrhodium rhodium dilio dilitho lithium 45 1803
Monday, September 6, 2021
09-06-2021-0134 - Pyrazole
Saturday, September 18, 2021
09-18-2021-0759 - Cyaphide SiR3-functionalised phosphaalkynes cyaphide complex Organometallic complexes of cyaphide were first reported in 1992 From 2-phosphaethynolate anion (−OC≡P) RuH(dppe) 2(CP) phosphaalkyne (P≡CH) Methylidynephosphane Phosphorus
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09-07-2021-1358 - Cyaphide
phosphorous/phosphorus, iodine, arsenic, acid
sulfur, silver, aluminum, lead
(mercury, argon)
boron, fluorine, beryllium
cesium, strontium
cobalt, uranium, graphite
cyanogen, cyanide
radon, og
antimony,
rhodium, ruthenium, lanathides, etc..
prussian blue Iron(II,III) hexacyanoferrate(II,III)
zero dipole, preon, neutronium, graviton, gauge, boson, baryon matter, pressuron, quantum interface, plasma, chain, cascade, zero state, residue, oscillation, vibration, glycerol, propylene glycol, cellulose, etc.. pressure, measure, ion, nuclear fusion, exotic, magnet, etc..
https://en.wikipedia.org/wiki/Periodic_table
https://en.wikipedia.org/wiki/Lanthanide_contraction
https://en.wikipedia.org/wiki/Allotropes_of_phosphorus#White_phosphorus
https://en.wikipedia.org/wiki/Potash
https://en.wikipedia.org/wiki/Phosphorus
https://en.wikipedia.org/wiki/Phosphorous
https://en.wikipedia.org/wiki/Phosphonium
https://en.wikipedia.org/wiki/Phosphine
https://en.wikipedia.org/wiki/Tetrakis(hydroxymethyl)phosphonium_chloride
https://en.wikipedia.org/wiki/Onium_ion
https://en.wikipedia.org/wiki/Zwitterion
https://en.wikipedia.org/wiki/Pnictogen
https://en.wikipedia.org/wiki/Ferrocyanide
https://en.wikipedia.org/wiki/Ylide
https://en.wikipedia.org/wiki/Magnetic_dipole
https://en.wikipedia.org/wiki/Magnetic_dipole–dipole_interaction
https://en.wikipedia.org/wiki/Zero-point_energy
https://en.wikipedia.org/wiki/Quadrupole
https://en.wikipedia.org/wiki/Overtone_band
https://en.wikipedia.org/wiki/water
https://en.wikipedia.org/wiki/Amphoterism
https://en.wikipedia.org/wiki/chalcogen
https://en.wikipedia.org/wiki/Allotropes_of_oxygen
https://en.wikipedia.org/wiki/Ozone
https://en.wikipedia.org/wiki/Cyclic_ozone
https://en.wikipedia.org/wiki/Epoxide
https://en.wikipedia.org/wiki/Carbon_tetraiodide
https://en.wikipedia.org/wiki/Triphenylphosphine
https://en.wikipedia.org/wiki/Desiccant
https://en.wikipedia.org/wiki/Hygroscopy
https://en.wikipedia.org/wiki/Dehydrogenation
https://en.wikipedia.org/wiki/hydrogenation
https://en.wikipedia.org/wiki/Protonation
https://en.wikipedia.org/wiki/Proton–proton_chain
https://en.wikipedia.org/wiki/L-shell
https://en.wikipedia.org/wiki/Hyperfine_structure
https://en.wikipedia.org/wiki/Dipole_repeller
https://en.wikipedia.org/wiki/Anomalous_magnetic_dipole_moment
https://en.wikipedia.org/wiki/Baryon_asymmetry
https://en.wikipedia.org/wiki/Missing_baryon_problem
https://en.wikipedia.org/wiki/Sterile_neutrino
https://en.wikipedia.org/wiki/Zero_field_splitting
https://en.wikipedia.org/wiki/Permeability_(electromagnetism)
https://en.wikipedia.org/wiki/Earth%27s_magnetic_field
https://en.wikipedia.org/wiki/Multipole_radiation
https://en.wikipedia.org/wiki/Hydrogen_line
https://en.wikipedia.org/wiki/Polarization_density
https://en.wikipedia.org/wiki/Chemical_bond
https://en.wikipedia.org/wiki/Cosmic_microwave_background
https://en.wikipedia.org/wiki/Muon#Anomalous_magnetic_dipole_moment
https://en.wikipedia.org/wiki/Nuclear_magnetic_resonance#Zero_field_NMR
https://en.wikipedia.org/wiki/Ferromagnetism
https://en.wikipedia.org/wiki/L-shell#Charged_particle_motions_in_a_dipole_field
https://en.wikipedia.org/wiki/Distributive_lattice
https://en.wikipedia.org/wiki/Ionization
https://en.wikipedia.org/wiki/Ionization#Quasi-static_tunnel_ionization
https://en.wikipedia.org/wiki/Above-threshold_ionization
https://en.wikipedia.org/wiki/Degree_of_ionization
https://en.wikipedia.org/wiki/Vacuum
https://en.wikipedia.org/wiki/pressure
https://en.wikipedia.org/wiki/temperature
https://en.wikipedia.org/wiki/volume
https://en.wikipedia.org/wiki/path
https://en.wikipedia.org/wiki/Radiation#Ionizing_radiation
https://en.wikipedia.org/wiki/Thermal_ionization
https://en.wikipedia.org/wiki/Resonance_ionization
https://en.wikipedia.org/wiki/Fast_atom_bombardment
https://en.wikipedia.org/wiki/Penning_ionization
https://en.wikipedia.org/wiki/Photoionization
https://en.wikipedia.org/wiki/Atmospheric-pressure_laser_ionization
https://en.wikipedia.org/wiki/Rydberg_atom
https://en.wikipedia.org/wiki/Atomic_radius
https://en.wikipedia.org/wiki/Exotic_atom
https://en.wikipedia.org/wiki/Grand_canonical_ensemble
https://en.wikipedia.org/wiki/Sample_preparation_in_mass_spectrometry#Matrix-assisted_laser_desorption%2Fionization
https://en.wikipedia.org/wiki/Electron
https://en.wikipedia.org/wiki/proton
https://en.wikipedia.org/wiki/neutron
https://en.wikipedia.org/wiki/Atom
High Energy Particle Physics,
https://en.wikipedia.org/wiki/Hypernucleus
https://en.wikipedia.org/wiki/Exciton
https://en.wikipedia.org/wiki/Electron_hole
https://en.wikipedia.org/wiki/Di-positronium
https://en.wikipedia.org/wiki/High_Energy_and_Particle_Physics_Prize
https://en.wikipedia.org/wiki/Positronium_hydride
https://en.wikipedia.org/wiki/Muonium_chloride
https://en.wikipedia.org/wiki/Quantum_chromodynamics
https://en.wikipedia.org/wiki/Electroweak_interaction
https://en.wikipedia.org/wiki/Asymptotic_freedom
https://en.wikipedia.org/wiki/Gauge_theory
https://en.wikipedia.org/wiki/Deep_inelastic_scattering
https://en.wikipedia.org/wiki/Rutherford_scattering
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