Carbamoyl phosphate synthetase I is a ligase enzyme located in the mitochondriainvolved in the production of urea. Carbamoyl phosphate synthetase I (CPS1 or CPSI) transfers an ammonia molecule from glutamine or glutamate to a molecule of bicarbonate that has been phosphorylated by a molecule of ATP. The resulting carbamate is then phosphorylated with another molecule of ATP. The resulting molecule of carbamoyl phosphate leaves the enzyme.
https://en.wikipedia.org/wiki/Carbamoyl_phosphate_synthetase_I
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