08-11-2021-0518 - [1984] TNF & Identical Pairing in Vitro (e.g. synthesis, manual structure/order/etc., sequencing, etc.) [1984]

1984.

The cDNAs encoding LT and TNF were cloned in 1984[23]

Gene[edit]

The human TNF gene was cloned in 1985.^[30] It maps to chromosome 6p21.3, spans about 3 kilobases and contains 4 exons. The last exon shares similarity with lymphotoxin alpha (LTA, once named as TNF-β).^[31] The three prime untranslated region (3'-UTR) of TNF contains an AU-rich element(ARE).

https://en.wikipedia.org/wiki/Tumor_necrosis_factor

Nature

. 1984 Dec 20-1985 Jan 2;312(5996):724-9.doi: 10.1038/312724a0.
Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin
D Pennica, G E Nedwin, J S Hayflick, P H Seeburg, R Derynck, M A Palladino, W J Kohr, B B Aggarwal, D V Goeddel

PMID: 6392892
DOI: 10.1038/312724a0

Abstract

Human tumour necrosis factor has about 30% homology in its amino acid sequence with lymphotoxin, a lymphokine that has similar biological properties. Recombinant tumour necrosis factor can be obtained by expression of its complementary DNA in Escherichia coli and induces the haemorrhagic necrosis of transplanted methylcholanthrene-induced sarcomas in syngeneic mice.

https://pubmed.ncbi.nlm.nih.gov/6392892/

Nature

. 1984 Dec 20-1985 Jan 2;312(5996):721-4. doi: 10.1038/312721a0.
Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity
P W Gray, B B Aggarwal, C V Benton, T S Bringman, W J Henzel, J A Jarrett, D W Leung, B Moffat, P Ng, L P Svedersky, et al.

PMID: 6334807
DOI: 10.1038/312721a0

A chemically-synthesized gene and natural complementary DNA coding for human lymphotoxin were isolated and engineered for expression in Escherichia coli. Purified recombinant lymphotoxin shows cytotoxic activity on murine and human tumour cell lines in vitro and causes necrosis of certain murine sarcomas in vivo.

https://pubmed.ncbi.nlm.nih.gov/6334807/